Anti-NQO1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P15559

Core Product Specifications and Parameters

Biological Background: NAD(P)H dehydrogenase [quinone] 1 (NQO1) Function and Localization

• NQO1 (NAD(P)H dehydrogenase [quinone] 1) is a flavin-containing enzyme that catalyzes the two-electron reduction of quinones to hydroquinones, using either NADH or NADPH as electron donors. The electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Related references: PMID:8999809 PMID:9271353

• The enzyme plays a central role in cellular redox regulation by detoxifying quinones and reducing components of the plasma membrane redox system, such as coenzyme Q and vitamin quinones, to produce antioxidant hydroquinone forms. It may function as a superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion. Related references: PMID:15102952 PMID:8999809 PMID:9271353

• NQO1 can also activate certain quinones and their derivatives, generating redox-reactive hydroquinones with potential DNA cross-linking and antitumor activities. Related references: PMID:8999809

• Upon oxidative stress, NQO1 interacts with tumor suppressors TP53 and TP73 in an NADH-dependent manner, inhibiting their ubiquitin-independent degradation by the 20S proteasome. Related references: PMID:15687255 PMID:28291250

• The protein is localized in the cytoplasm and cytosol.

• NQO1 is a phosphoprotein subject to post-translational modifications, including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity and stability.

• Alternative splicing of the NQO1 gene generates multiple transcript variants, potentially giving rise to different protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human NQO1 sequence (amino acids 1–274), so the antibody may recognize both native and denatured protein forms in applications such as WB, IF/ICC, and ELISA.
• For WB, consider using a standard reducing denaturing gel and validate the detected band at approximately 31 kDa using a relevant cell or tissue lysate known to express NQO1 (e.g., liver, kidney, or cancer cell lines).
• In IF/ICC, include appropriate permeabilization and fixation steps to ensure access to the cytosolic target; a nuclear counterstain can help visualize localization.
• Titrate the antibody for each application and biological system, and include proper positive and negative controls to confirm specificity, especially given the potential for multiple isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutions, and laboratories with a streamlined supply chain for IVD raw materials. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting projects in redox biology, oncology, and beyond. For NQO1-related research and assay development, our antibody portfolio and technical resources help accelerate your progress from concept to clinic.