Anti-NQO1 Rabbit Monoclonal Antibody (KO Validated) for WB, IF-P, ELISA - P15559

Core Product Specifications and Parameters

Biological Background: NQO1 Function and Localization

• NQO1, also known as DT-diaphorase (DTD), quinone reductase 1 (QR1), and menadione reductase, is a flavin-containing oxidoreductase.
• It catalyzes the two-electron reduction of quinones to hydroquinones using NADH or NADPH, bypassing semiquinone radicals and reactive oxygen species generation. Related references: PMID:8999809, PMID:9271353
• NQO1 plays a critical role in cellular redox homeostasis by detoxifying quinones and reducing components of the plasma membrane redox system such as coenzyme Q and vitamin quinones. Related references: PMID:15102952, PMID:8999809, PMID:9271353
• Alternatively, NQO1 can bioactivate certain quinones, generating redox-active hydroquinones that exhibit DNA cross-linking and antitumor potential. Related references: PMID:8999809
• NQO1 acts as a gatekeeper of the 20S proteasome, interacting with tumor suppressors TP53 and TP73 in an NADH-dependent manner to inhibit their ubiquitin-independent degradation. Related references: PMID:15687255, PMID:28291250
• The protein is predominantly localized to the cytoplasm and cytosol.
• NQO1 undergoes alternative splicing and post-translational modifications including phosphorylation and ubiquitination.
• It is a flavoprotein requiring FAD as a cofactor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100); it may recognize multiple isoforms or splice variants.
• For western blotting, the predicted molecular weight is ~31 kDa; consider loading appropriate controls and using knockout or knockdown lysates for specificity validation.
• For immunofluorescence staining of paraffin-embedded sections (IF-P), optimize antigen retrieval and antibody dilution; note that NQO1 exhibits cytoplasmic localization.
• For ELISA applications, pair with a suitable capture or detection antibody and validate performance in the relevant biological matrix.
• Cross-reactivity has been reported for human, mouse, and rat; verify sequence homology if extending to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing platform for IVD raw materials, technical services, and consultancy. This KO-validated anti-NQO1 rabbit monoclonal antibody, suited for redox biology and cancer research, exemplifies our commitment to supplying high-quality antibodies, validated pairs, bulk ancillary reagents, and rare-target reagents. From concept to clinic, we streamline your procurement and accelerate assay development.