Anti-NQO1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15559

Core Product Specifications and Parameters

Biological Background: NQO1 Function and Localization

• NQO1 is a flavin-containing quinone reductase that catalyzes the two-electron reduction of quinones to hydroquinones, using NADH or NADPH as electron donors, thereby bypassing harmful semiquinone and reactive oxygen species formation (By similarity) (PubMed:8999809, PubMed:9271353). Related references: PMID:8999809 PMID:9271353

• It regulates cellular redox state by reducing components of the plasma membrane redox system (e.g., coenzyme Q, vitamin quinones) to antioxidant hydroquinones and may act as a superoxide scavenger (PubMed:15102952, PubMed:8999809, PubMed:9271353). Related references: PMID:15102952 PMID:8999809 PMID:9271353

• Conversely, NQO1 can activate certain quinones and derivatives, generating redox reactive hydroquinones with potential DNA cross-linking and antitumor effects (PubMed:8999809). Related references: PMID:8999809

• Under oxidative stress, NQO1 serves as a gatekeeper of the core 20S proteasome by interacting with TP53 and TP73 in a NADH-dependent manner, inhibiting their ubiquitin-independent degradation (PubMed:15687255, PubMed:28291250). Related references: PMID:15687255 PMID:28291250

• The enzyme localizes primarily to the cytoplasm/cytosol (SUBCELLULAR LOCATION).

• NQO1 is subject to alternative splicing, phosphorylation, and ubiquitin-like conjugation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human NQO1 (1-274), suggesting the antibody may recognize multiple epitopes; consider using peptide blocking or knockout controls to confirm specificity.
• For western blotting, the predicted molecular weight is 31 kDa; validate in relevant cell or tissue lysates; note that post-translational modifications may cause slight band shifts.
• In immunohistochemistry (paraffin), antigen retrieval conditions should be optimized due to cytoplasmic localization of NQO1.
• For immunofluorescence/ICC, a cytosolic staining pattern is expected; include appropriate fixation and permeabilization steps.
• In ELISA applications, this polyclonal antibody may be used as capture or detection reagent; pairing with a matched monoclonal is recommended for quantitative sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including thoroughly optimized monoclonal and polyclonal antibodies. For redox biology and cancer research, we offer ready-to-use NQO1 reagents and can assist in sourcing rare target antibodies, bulk buffers, and ancillary biochemicals. Whatever your development pipeline requires—from early feasibility to large-scale production—CamelBio bridges the gap between concept and clinic.