Anti-NPY5R Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q15761

Core Product Specifications and Parameters

Biological Background: NPY5R Function and Localization

• NPY5R, also known as NPY-Y5 receptor or NPYY5-R, is a receptor for neuropeptide Y and peptide YY.
• It is a G protein-coupled receptor (GPCR) that mediates signals by inhibiting adenylate cyclase activity through G proteins.
• NPY5R is primarily associated with the regulation of food intake and may play a role in feeding disorders.
• The receptor is located on the cell membrane and is expressed predominantly in the brain, specifically the hypothalamus.
• Key structural features include disulfide bonds, glycosylation sites, and palmitoylation, which are typical of GPCRs.
• It belongs to the transmembrane receptor family, possessing seven transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), which is predicted to be extracellular, likely providing good accessibility for antibody binding in native conditions.
• For Western blotting, a band around 51 kDa is expected; consider using relevant positive control lysates from brain or hypothalamus.
• In immunofluorescence/ICC, membrane localization should be observed; validate staining patterns in known NPY5R-expressing cell lines.
• This antibody may be suitable for ELISA-based detection of NPY5R; titrate antibody concentration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material supply solutions, including validated monoclonal antibodies like this anti-NPY5R antibody, ideal for neuroscience and metabolic disorder research. We offer one-stop access to antibody pairs, ancillary reagents, and custom sourcing for rare targets, supporting diagnostic manufacturers from concept to clinic.