Anti-NPY4R Rabbit Polyclonal Antibody for WB, ELISA - P50391

Core Product Specifications and Parameters

Biological Background: NPY4R Function and Localization

• NPY4R (Y4, PP1, PPYR1) is a G protein-coupled receptor that binds pancreatic polypeptide (PP), neuropeptide Y (NPY), and peptide YY (PYY).
• The receptor is negatively coupled to adenylate cyclase, inhibiting cAMP production, with a rank order of affinity: PP > [Pro-34] PYY > PYY > NPY. Related references: PMID:7493937 PMID:7592911
• NPY4R is predominantly located at the cell membrane.
• Tissue expression is highest in the brain, coronary artery, and ileum, with lower levels in pancreas and kidney, and it is also detected in colon and small intestine.
• The receptor undergoes post-translational modifications including glycosylation, palmitoylation, and contains a disulfide bond, and its 3D structure has been resolved.
• Its molecular weight is approximately 42 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 1-80), making the antibody suitable for detecting both glycosylated and unglycosylated receptor forms.
• For Western blot, consider using membrane-enriched protein extracts and validate in human tissue or cell lines expressing NPY4R.
• For ELISA, optimize coating conditions using purified recombinant protein or cell lysates.
• As cross-reactivity is reported only for human, verify specificity in your sample system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies, antibody pairs, and ancillary reagents, to support neuropeptide receptor research and diagnostic development. For this NPY4R antibody, we ensure reliable supply and can assist with technical consulting, custom sourcing, and bulk requirements. Partner with CamelBio to accelerate your project from concept to clinic.