Anti-NPY Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01303

Core Product Specifications and Parameters

Biological Background: NPY Function and Localization

• Pro-neuropeptide Y (NPY) is a member of the neuropeptide Y family, produced from a precursor protein through post-translational cleavage into active peptides, including the mature NPY hormone.
• NPY acts as a ligand for neuropeptide Y receptor type 1 (NPY1R) and type 2 (NPY2R). Receptor binding triggers adenylate cyclase-inhibiting G protein-coupled receptor signaling, resulting in decreased cAMP production, and also increases intracellular Ca(2+) levels. Related references: PMID:1321422, PMID:7592910, PMID:35165283, PMID:38882210
• NPY is involved in the regulation of eating behavior, acting as a potent stimulator of food intake.
• In the gastrointestinal system, NPY contributes to the regulation of colonic antisecretory tone and contractility.
• Subcellularly, NPY is secreted and stored in cytoplasmic vesicles, particularly in neuronal dense core vesicles.
• One of the most abundant peptides in the nervous system, NPY is also found in some chromaffin cells of the adrenal medulla.
• The precursor undergoes essential post-translational modifications, including amidation and cleavage at pairs of basic residues, to yield the bioactive neuropeptide.

Experimental Guidance and Technical Tips

• As a polyclonal antibody, this reagent may recognize multiple epitopes on the target protein; consider performing validation in the specific sample type and assay system of interest.
• For Western blot analysis, a band around 11 kDa corresponding to the pro-neuropeptide Y precursor is expected; mature NPY (~4 kDa) may also be detected in some samples.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and antibody dilution as protocols can vary across tissues and fixation methods.
• In immunofluorescence (IF/ICC), employ appropriate permeabilization to detect NPY localized in cytoplasmic vesicles; include controls to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical services, covering every stage from concept to clinic. For NPY-related research and assay development, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials. Our solutions are designed to accelerate your project while maintaining rigorous quality standards.