Anti-NPTX2 Rabbit Polyclonal Antibody for WB and ELISA - P47972

Core Product Specifications and Parameters

Biological Background: NPTX2 Function and Localization

• NPTX2 encodes neuronal pentraxin-2 (NP-II), also known as NARP or NP2, a member of the pentraxin family characterized by a conserved pentraxin domain.
• It is likely involved in the modification of cellular properties underlying long-term plasticity, a key process in learning and memory formation.
• Neuronal pentraxin-2 binds to the agar matrix in a calcium-dependent manner, indicating lectin-like activity that may mediate extracellular interactions.
• The protein is secreted and features a signal peptide, disulfide bonds, and glycosylation sites, consistent with a role in the synaptic cleft or extracellular matrix.
• Tissue expression is broad, with the highest levels detected in testis; it is also present in brain, pancreas, liver, heart, and skeletal muscle.
• As a calcium-binding lectin, NPTX2 may participate in organizing synaptic components and modulating receptor clustering or synapse remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 90–240 of human NPTX2; verify antibody specificity in your target tissue or cell line using siRNA knockdown or antigen competition.
• For Western blot, the predicted molecular weight is approximately 47 kDa; confirm band identity with known standards and consider that post-translational modifications may shift migration.
• Optimize antibody dilution and incubation conditions empirically for both WB and ELISA; include appropriate positive (e.g., recombinant protein or overexpressing lysate) and negative controls.
• This antibody reacts with human NPTX2; cross-reactivity with other species should be validated independently if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with consolidated access to high-quality IVD raw materials, including antibodies, antigens, and ancillary reagents. For investigations into synaptic plasticity and neuronal pentraxins, we supply validated reagents and offer sourcing support for rare targets to help streamline your assay development from concept to clinical application.