Anti-NPSR1 Rabbit Polyclonal Antibody for WB and ELISA - Q6W5P4

Core Product Specifications and Parameters

Biological Background: NPSR1 Function and Localization

• NPSR1 encodes the Neuropeptide S receptor, also known as G protein-coupled receptor 154 (GPR154), GPRA, or PGR14, a typical seven-transmembrane GPCR.
• Receptor activation by neuropeptide S (NPS) initiates dual signaling: Gq-dependent phospholipase C activation mobilizes intracellular Ca²⁺, and Gs-dependent stimulation elevates cAMP levels (PubMed:15312648, PubMed:15947423, PubMed:16720571, PubMed:26865629). Related references: PMID:15312648 PMID:15947423 PMID:16720571 PMID:26865629
• Both Ca²⁺ and cAMP pathways converge on ERK1/2 phosphorylation, and NPS binding ultimately inhibits cell growth (PubMed:15947423, PubMed:26865629). Related references: PMID:15947423 PMID:26865629
• Alternative splicing of NPSR1 generates multiple isoforms with distinct expression profiles: isoform 1 is predominant in smooth muscle, whereas isoform 4 is ubiquitous in epithelial tissues, including bronchus, stomach, intestine, and uterus (PubMed:15947423). Related references: PMID:15947423
• Subcellularly, NPSR1 is found at the cell membrane and can cycle to the cytoplasm, a common feature of activated GPCRs.
• NPSR1 expression is significantly upregulated in the bronchial smooth muscle and epithelium of asthma patients, linking this receptor to asthma pathophysiology (PubMed:15947423). Related references: PMID:15947423
• Key post-translational modifications include N-linked glycosylation and conserved disulfide bonds, critical for receptor folding and ligand binding.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the C-terminal region (aa 200–C-terminus) of human NPSR1 isoform 1 (NP_001287863.1). This region is intracellular and may be conserved across splice variants; verify reactivity with your specific isoform of interest.
• For Western blotting, a band around 43 kDa is expected for the full-length receptor, but glycosylation or dimerization may produce larger molecular weight species. Validate antibody specificity in NPSR1-overexpressing versus knockdown/knockout cell models.
• In ELISA applications, direct coating of the immunogen peptide or recombinant NPSR1 extracellular domain may be suitable. Optimize coating conditions and include negative controls.
• Given the receptor’s expression in smooth muscle and epithelia, consider using lysates from bronchial smooth muscle cells, colon, or uterus for initial testing.
• Since NPSR1 is a GPCR, membrane protein extraction protocols are recommended for optimal detection by WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents for immunodiagnostic assay development. This anti-NPSR1 polyclonal antibody is part of our expanding catalog of GPCR-targeting reagents, supporting asthma and respiratory disease research. From concept to clinic, CamelBio connects diagnostic developers with high-quality, cost-effective raw materials and technical consultation.