Anti-NPRL2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WTW4

Core Product Specifications and Parameters

Biological Background: NPRL2 Function and Localization

• NPRL2 (also known as NPR2, NPR2L, TUSC4, FFEVF2) is a catalytic component of the GATOR1 complex, which acts as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. Related references: PMID:23723238, PMID:29590090, PMID:35338845
• Upon amino acid deprivation, GATOR1 acts as a GTPase-activating protein (GAP) for RagA/RRAGA or RagB/RRAGB, converting them to the inactive GDP-bound state, leading to mTORC1 release from lysosomes and pathway inhibition. Related references: PMID:23723238, PMID:29590090, PMID:35338845
• When amino acids are abundant, GATOR2 ubiquitinates and inhibits GATOR1, allowing mTORC1 activation; additionally, PRMT1 methylates NPRL2 under methionine-sufficient conditions, inhibiting its GAP activity. Related references: PMID:23723238, PMID:36528027, PMID:27173016, PMID:30651352, PMID:35338845
• NPRL2 functions as a tumor suppressor by suppressing Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling, thereby inhibiting cell growth and enhancing sensitivity to anticancer drugs. Related references: PMID:18616680
• Subcellularly, NPRL2 localizes to the lysosome membrane, where it exerts its regulatory role in mTORC1 signaling.
• Tissue expression is highest in skeletal muscle, followed by brain, liver and pancreas, with lower levels in lung, kidney, placenta and heart; it is also expressed in various brain regions and most lung cancer cell lines. Related references: PMID:26505888, PMID:27173016
• NPRL2 is associated with disease variants linked to epilepsy and is subject to alternative splicing and post-translational modifications including methylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1–220 of human NPRL2 (NP_006536.3). This N-terminal region may be suitable for detecting full-length NPRL2 across species.
• Validated applications include Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Cross-reactivity with human, mouse, and rat samples has been reported; consider species-specific validation for your experimental system.
• For WB, a band at approximately 44 kDa is expected. Due to the polyclonal nature, additional non-specific bands may be observed; optimization of blocking and antibody dilution is recommended.
• When using IF/ICC, appropriate fixation and permeabilization methods should be tested, as NPRL2 localizes to lysosomal membranes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive sourcing solution for IVD raw materials, including validated polyclonal and monoclonal antibodies targeting key signaling molecules like NPRL2. Whether you require antibody pairs for sandwich ELISA, bulk ancillary reagents, or support for rare target raw-material acquisition, our platform bridges the gap from early development to clinical application, ensuring reliable supply for your diagnostic pipeline.