Anti-NPR3 (ANP-C) Rabbit Polyclonal Antibody for WB, ELISA - P17342

Core Product Specifications and Parameters

Biological Background: NPR3 Function and Localization

• Encodes the atrial natriuretic peptide receptor 3 (NPR3), also known as the clearance receptor (NPR-C / ANP-C), which binds natriuretic peptides NPPA (ANP), NPPB (BNP), and NPPC (CNP) with comparable affinity.
• Functions primarily as a clearance receptor, internalizing and degrading circulating natriuretic peptides to regulate their local concentrations and downstream signaling effects.
• Acts as a modulator of bone growth and osteoblast differentiation by binding the ligand osteocrin; this interaction prevents natriuretic peptide binding and leads to increased cGMP production.
• Localizes to the cell membrane as a single-pass transmembrane protein, with a large extracellular ligand-binding domain and a short cytoplasmic tail.
• Contains structural features typical of the natriuretic peptide receptor family, including a signal peptide, multiple disulfide bonds, and N-linked glycosylation sites.
• Alternative splicing generates multiple transcript variants, and mutations in this gene have been associated with disease phenotypes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 272–541 of human NPR3 (isoform NP_001191304.1), which covers a portion of the extracellular domain; consider this when designing detection/blocking strategies.
• For Western blot, a band near 60 kDa is expected; we recommend titrating the antibody (e.g., 1:500–1:2000) and validating it in relevant human, mouse, or rat tissue lysates.
• In ELISA applications, the polyclonal nature may provide enhanced sensitivity; consider pairing with a monoclonal capture antibody if a sandwich format is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a comprehensive, one-stop sourcing platform for IVD raw materials, covering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research involving natriuretic peptide signaling and NPR3, we can connect you with tailored raw material solutions to support assay development and scale-up. Our B2B approach streamlines your supply chain from concept to clinic.