Anti-NPM3 Polyclonal Antibody for WB, ELISA - O75607

Core Product Specifications and Parameters

Biological Background: NPM3 Function and Localization

• NPM3 acts as a histone chaperone and is involved in ribosome biogenesis, chromatin remodeling, and protein chaperoning. Related references: PMID:20073534, PMID:22362753
• It modulates the histone chaperone function and RNA-binding activity of nucleolar phosphoprotein B23/NPM1. Related references: PMID:22362753
• NPM3 forms heteropentamers with NPM1 to efficiently mediate chromatin remodeling. Related references: PMID:15596447
• Subcellular localization is predominantly in the nucleus and nucleolus.
• Ubiquitously expressed in human tissues.
• Post-translational modifications include acetylation, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 30–140) covers a central region of NPM3; consider its potential to detect full-length and any relevant isoforms.
• For Western blot, a band at approximately 19 kDa is expected; optimize blocking and antibody dilution for your specific sample type.
• In ELISA, validate the antibody’s performance using recombinant NPM3 or appropriate cell lysates under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This NPM3 antibody supports chromatin and nucleolar research, and our team can assist with rare target sourcing and custom reagent development. Contact us to streamline your diagnostic or research workflow from concept to clinic.