Anti-NPLOC4 Rabbit Polyclonal Antibody for WB - Q8TAT6

Core Product Specifications and Parameters

Biological Background: NPLOC4 Function and Localization

• NPLOC4 (Nuclear Protein Localization Protein 4 Homolog), also known as NPL4, is a component of the UFD1-VCP-NPLOC4 ternary complex that recognizes ubiquitinated proteins and mediates their export from the endoplasmic reticulum to the cytoplasm for proteasomal degradation.
• The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is required for closed nuclear envelope formation (By similarity).
• Acts as a negative regulator of type I interferon production by forming a complex with VCP and UFD1 that binds RIGI, recruiting RNF125 to promote ubiquitination and degradation of RIGI. Related references: PMID:26471729
• Localizes to the cytoplasm, cytosol, endoplasmic reticulum, and nucleus, supporting roles in both ER-associated degradation and nuclear processes.
• Highest expression is observed in brain, heart, skeletal muscle, kidney, and fetal liver.
• Contains a zinc-finger domain and undergoes acetylation; also subject to alternative splicing. Functional keywords include chaperone, Ubl conjugation pathway, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-270) of human NPLOC4, which may provide specificity for native protein detection in multiple species.
• For Western blot (WB), expect a band near 68 kDa; consider using positive lysates from brain or heart tissues where NPLOC4 is highly expressed.
• In immunocytochemistry/immunofluorescence (IF/ICC), optimize fixation and permeabilization to detect NPLOC4 in its various subcellular locations (ER, cytosol, nucleus).
• ELISA can be used as a quantitative platform; ensure appropriate coating and detection conditions for polyclonal antibodies.
• Validate antibody performance in your specific sample type, as cross-reactivity with mouse and rat has been reported but may vary in different applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a complete selection of IVD raw materials, including well-characterized antibodies for the ubiquitin-proteasome system and ER-associated degradation pathways. We supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare targets like NPLOC4 to streamline your assay development—from concept to clinic. Let our expertise bridge your procurement needs for reliable, high-performance immunoreagents.