Anti-NPL Rabbit Polyclonal Antibody for WB, ELISA - Q9BXD5

Core Product Specifications and Parameters

Biological Background: NPL Function and Localization

• N-acetylneuraminate lyase (NPL), also known as sialic acid aldolase or sialate lyase, is a cytoplasmic enzyme encoded by the NPL gene. It belongs to the lyase family and catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to pyruvate and N-acetylmannosamine via a Schiff base intermediate. Related references: PMID:33895133
• By breaking down sialic acids, NPL prevents their recycling back to the cell surface, playing a crucial role in regulating sialic acid levels on glycoproteins and glycolipids. Related references: PMID:33895133
• NPL is involved in the degradation pathway of N-glycolylneuraminic acid (Neu5Gc), a non-human sialic acid obtained from the diet that must be cleared from the body. Related references: PMID:22692205, PMID:33895133
• The protein localizes to the cytoplasm and is expressed in multiple tissues; isoform 2 is specifically found in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine, and peripheral blood leukocytes.
• The mature protein has a molecular weight of approximately 35 kDa, consistent with the product specification of 35kDa.
• Alternative splicing generates multiple transcript variants, and the enzyme functions via a Schiff base intermediate, classified under carbohydrate metabolism and lyase activity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-250 of human NPL; this N-terminal region may be useful for detecting full-length NPL in WB. Consider reduced denaturing conditions given the expected band near 35 kDa.
• Cross-reactivity with mouse and rat suggests the antibody may be suitable for studies in rodent models; validate expression in relevant tissues such as liver or kidney where NPL is known to be expressed.
• For ELISA, the polyclonal nature may provide robust signal capture; however, titration and validation in the relevant sample matrix (e.g., cell lysates, tissue homogenates) are essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw-material solutions, connecting diagnostic manufacturers and research institutes with high-quality reagents. This anti-NPL antibody supports sialic acid metabolism research, and our supply capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your development pipeline. Whether you need technical consultation or procurement support, CamelBio is your single point of contact from concept to clinic.