Anti-NPHS2 Rabbit Monoclonal Antibody for WB and ELISA - Q9NP85

Core Product Specifications and Parameters

Biological Background: Podocin Function and Localization

• Podocin is a 42 kDa protein encoded by the NPHS2 gene, with alternative splicing variants reported. It is a key structural component of the glomerular slit diaphragm in kidney podocytes.
• In glomerular podocytes, podocin acts as a linker between the plasma membrane and the actin cytoskeleton, playing an essential role in the regulation of glomerular permeability and ultrafiltration.
• Podocin is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, highlighting its specialized role in renal filtration.
• Subcellularly, podocin localizes to the cell membrane and endoplasmic reticulum, where it may contribute to membrane-cytoskeleton anchoring and signaling.
• Post‑translational modifications include palmitoylation and N‑glycosylation; palmitate attachment likely facilitates membrane association and lipid‑raft targeting.

Experimental Guidance and Technical Tips

• The immunogen was designed against the C‑terminal region (amino acids 284–383), which may be accessible in detergent‑solubilized lysates; for WB, consider using reducing conditions and a 10% SDS‑PAGE gel to resolve the 42 kDa band.
• Based on the application list, WB and ELISA are suggested; for WB, validate the antibody in human podocyte cell lines (e.g., AB8/13) or kidney glomerular extracts, and for ELISA, use recombinant podocin or the synthetic peptide as the coating antigen.
• Cross‑reactivity is reported as human; if using other species, verify sequence homology in the immunogen region before testing.

CamelBio: Your One ‑Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw‑material partner, offering this anti‑podocin rabbit monoclonal antibody for kidney biomarker research and diagnostic assay development. Our portfolio includes validated antibody pairs, bulk recombinant monoclonal antibodies, and ancillary reagents, with the capability to source rare‑target raw materials to support your entire workflow from discovery to clinical application.