Anti-NPHS2 Polyclonal Antibody for WB, ELISA - Q9NP85

Core Product Specifications and Parameters

Biological Background: NPHS2 (Podocin) Function and Localization

• NPHS2 encodes podocin, a key component of the slit diaphragm complex in kidney podocytes.
• Podocin acts as a scaffolding protein, linking the plasma membrane to the cytoskeleton, and is critical for maintaining the glomerular filtration barrier integrity.
• The protein is almost exclusively expressed in podocytes of fetal and mature kidney glomeruli, underscoring its kidney-specific function.
• Subcellularly, podocin localizes to the cell membrane and endoplasmic reticulum, consistent with its role in membrane-cytoskeleton linkage.
• Post-translational modifications include palmitoylation and glycosylation, which may regulate its membrane association and stability.
• NPHS2 is associated with disease variants; mutations lead to steroid-resistant nephrotic syndrome, highlighting its clinical significance.
• Alternative splicing of NPHS2 produces isoforms that may have distinct functional roles.
• Keywords such as lipoprotein and proteomics identification suggest involvement in lipid interactions and broad experimental detection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa300-388), making it suitable for detecting full-length podocin and potentially certain isoforms.
• For Western blot, consider using podocin-enriched kidney lysates from human, mouse, or rat, as podocin expression is restricted to podocytes.
• The predicted molecular weight is ~42 kDa; note that glycosylation and other modifications may cause slight band shifts.
• In ELISA applications, optimize peptide coating or capture conditions to account for the polyclonal nature of the antibody.
• Since cross-reactivity includes mouse and rat, this antibody can be used in widely adopted rodent models of kidney disease.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to premium IVD raw materials like this anti-NPHS2 antibody, which supports renal biomarker development and glomerular disease research. Beyond validated antibody pairs and optimized polyclonal/monoclonal reagents, we provide bulk ancillary reagents and can source rare-target raw materials to accelerate your projects. From concept to clinic, rely on CamelBio’s integrated supply network and technical expertise for all your IVD needs.