Anti-NPHP4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75161

Core Product Specifications and Parameters

Biological Background: Nephrocystin-4 Function and Localization

• Nephrocystin-4 (also known as nephroretinin) is encoded by the NPHP4 gene; multiple isoforms are produced through alternative splicing.
• Involved in the organization of apical junctions and ciliary function; proposed to operate within a NPHP1-4-8 module. Required for building functional cilia and contributes to organizing the subapical actin network in multiciliated epithelial cells. Related references: PMID:19755384 PMID:21565611
• Cooperates with INVS to down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating disheveled proteins. Stabilizes JADE1 and promotes its nuclear translocation, leading to cooperative inhibition of canonical Wnt signaling. Related references: PMID:21498478 PMID:22654112
• Acts as a negative regulator of the Hippo pathway by associating with LATS1 and modulating LATS1-dependent phosphorylation and localization of WWTR1/TAZ. Related references: PMID:21555462
• Subcellular localization includes the cytoplasm, cilium basal body, centrosome, tight junctions, and nucleus, consistent with its roles in ciliary and junctional functions.
• Predominantly expressed in kidney, skeletal muscle, heart, and liver; lower expression is found in brain and lung.
• Phosphorylated in cells, which may influence its signaling activities. Mutations in NPHP4 are associated with nephronophthisis, Senior-Loken syndrome, and Leber congenital amaurosis, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (amino acids 1247–1426). Verify reactivity when working with isoforms or splice variants that may differ in this domain.
• Validated for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA with human, mouse, and rat samples. If using other species, perform a cross‑reactivity pilot experiment.
• For WB, the predicted molecular weight is ~158 kDa; use a low‑percentage acrylamide gel and extended transfer times to ensure good resolution and transfer of high‑molecular‑weight proteins.
• In IF/ICC, target localization includes cilia and tight junctions. Optimize fixation and permeabilization conditions to preserve these structures and epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to high‑quality IVD raw materials. This anti‑NPHP4 antibody supports investigation of ciliopathies and renal diseases, and CamelBio can additionally supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare‑target raw materials to move your projects efficiently from concept to clinic.