Anti-NPHP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15259

Core Product Specifications and Parameters

Biological Background: Nephrocystin-1 Function and Localization

• Nephrocystin-1 (NPHP1) is a protein encoded by the NPHP1 gene, also known as juvenile nephronophthisis 1 protein; alternative splicing produces multiple isoforms.
• It plays a critical role in epithelial cell polarity by interacting with BCAR1 and organizing apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8.
• NPHP1 is involved in the regulation of intraflagellar transport (IFT) during cilia assembly, though it is not strictly required for ciliogenesis.
• The protein is required for normal retina development and influences the movement of IFT proteins such as IFT88 and WDR19 in connecting photoreceptor cilia.
• Subcellularly, nephrocystin-1 localizes to cell junctions (adherens and tight junctions), cilia (including axoneme), and the cytoplasm.
• It is classified as a phosphoprotein and contains an SH3 domain and coiled coil region; keywords include ciliopathy, differentiation, and spermatogenesis.
• Tissue expression is widespread, with highest levels in pituitary gland, spinal cord, thyroid, testis, skeletal muscle, lymph node, and trachea; it is also expressed in nasal epithelial cells and renal collecting duct.
• Mutations in NPHP1 are associated with ciliopathies such as nephronophthisis, Joubert syndrome, Leber congenital amaurosis, and Senior-Loken syndrome.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 463–732 of human NPHP1 (isoform NP_997064.2), a region that may provide specificity; consider sequence alignment with related family members.
• For Western blotting, a band around 83 kDa is expected; validate using appropriate positive controls (e.g., testis or kidney lysates) and account for potential isoforms.
• This polyclonal antibody may detect multiple isoforms or post-translationally modified forms; titrate antibody concentration for IHC-P and IF/ICC to reduce background.
• Cross-reactivity with mouse and rat has been reported, but always confirm species-specific reactivity in your experimental system through parallel testing.
• For ELISA applications, using recombinant or captured protein may help assess antibody affinity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, to support diagnostic manufacturers and research laboratories. For targets like NPHP1 involved in ciliopathies and kidney diseases, CamelBio offers one-stop access to critical reagents and sourcing solutions tailored to accelerate your assay development from concept to clinic. Contact us to explore how our supply chain expertise can meet your specific raw-material requirements.