Anti-NPFF Polyclonal Antibody for WB, ELISA - O15130

Core Product Specifications and Parameters

Biological Background: NPFF Function and Localization

• NPFF (Pro-FMRFamide-related neuropeptide FF), also known as FMRFamide-related peptides, is a member of the RFamide peptide family encoded by the NPFF gene.
• It functions as a morphine-modulating peptide, influencing pain perception and opioid-induced analgesia.
• NPFF has diverse physiological effects, including elevation of arterial blood pressure and increased somatostatin secretion from the pancreas.
• The neuropeptide potentiates and sensitizes acid-sensing ion channels ASIC1 and ASIC3.
• The protein is secreted and undergoes post-translational processing such as amidation and cleavage on pairs of basic residues.
• Alternative splicing generates multiple isoforms of the precursor protein.

Experimental Guidance and Technical Tips

• Given the immunogen design targeting amino acids 34-113 of human NPFF, the antibody is expected to recognize the NPFF precursor and potentially its processed peptides. Validate reactivity in the relevant sample system.
• For Western blotting, consider starting with standard dilution ranges (e.g., 1:500–1:2000) and optimize based on sample type and expression levels.
• The antibody is suitable for ELISA; include appropriate controls and perform titration to determine optimal working concentration.
• Cross-reactivity is reported with human and rat; verify specificity in the chosen species if extended to additional ones.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-NPFF polyclonal antibody supports research in neuropeptide function, pain modulation, and related signaling pathways. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.