Anti-NPC2 Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P61916

Core Product Specifications and Parameters

Biological Background: NPC2 Function and Localization

• NPC2, also known as epididymal secretory protein E1 (HE1), is a small glycoprotein critical for intracellular cholesterol trafficking and lysosomal cholesterol export.
• NPC2 acts as an intracellular cholesterol transporter, facilitating cholesterol egress from lysosomes in concert with NPC1. Related references: PMID:11125141 PMID:15937921 PMID:17018531
• Unesterified cholesterol derived from LDLs is transferred by NPC2 to the N-terminal cholesterol-binding pocket of NPC1 for subsequent export. Related references: PMID:17018531 PMID:18772377 PMID:27238017
• NPC2 binds cholesterol with a 1:1 stoichiometry and can also interact with various sterols, including plant sterols such as stigmasterol and β-sitosterol. Related references: PMID:17018531
• A secreted form of NPC2 regulates biliary cholesterol secretion through stimulation of ABCG5/ABCG8-mediated cholesterol transport.
• NPC2 is predominantly localized to lysosomes and the endoplasmic reticulum, and is also secreted into extracellular fluids.
• NPC2 is expressed in a broad range of tissues, with high levels in gallbladder bile, kidney, liver, epididymis, and testis.
• Mutations in NPC2 are associated with Niemann-Pick disease type C, a lysosomal storage disorder characterized by cholesterol accumulation.

Experimental Guidance and Technical Tips

• The antibody is raised against full-length recombinant human NPC2 protein; optimal performance is expected in western blot detecting the ~17 kDa band.
• For western blot, consider using a 12% or 15% SDS-PAGE gel to resolve the 17 kDa NPC2 protein.
• Immunofluorescence and immunohistochemistry of endogenous NPC2 may require permeabilization and antigen retrieval steps; validate the protocol in your model system.
• The antibody cross-reacts with mouse and rat NPC2, enabling multi-species studies; sequence alignment should be verified if using in other species.
• ELISA applications can be developed using this monoclonal antibody as a capture or detection reagent; pair with a suitable detection antibody for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated monoclonal and polyclonal antibodies for cholesterol metabolism and Niemann-Pick disease research. Our sourcing solutions cover everything from optimized antibody pairs and bulk ancillary reagents to custom rare-target raw material procurement. With a streamlined supply chain and technical consulting, CamelBio accelerates your assay development from concept to clinic.