Anti-NPC2 (HE1, EDDM1) Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P61916

Core Product Specifications and Parameters

Biological Background: NPC2 Function and Localization

• NPC2 (also known as HE1, EDDM1) is an intracellular cholesterol transporter that plays a critical role in the egress of cholesterol from the lysosomal compartment.
• It acts in concert with NPC1; cholesterol released from LDLs in late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket of NPC1.
  Related references:
  PMID:17018531 PMID:18772377 PMID:27238017
• NPC2 binds cholesterol with 1:1 stoichiometry and can also bind a variety of other sterols, including plant sterols.
  Related references:
  PMID:17018531
• The secreted form of NPC2 regulates biliary cholesterol secretion through stimulation of ABCG5/ABCG8-mediated transport.
• Subcellularly, NPC2 is found in the lysosome, endoplasmic reticulum, and is secreted.
• It is detected in multiple tissues, including gallbladder bile, fibroblasts, kidney, liver, spleen, small intestine, placenta, testis, and epididymis.
• The protein contains a signal peptide and features post-translational modifications such as disulfide bonds, glycosylation, and acetylation; mutations in NPC2 are linked to Niemann-Pick disease type C2.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 20–151 of human NPC2, which corresponds to the full mature protein after signal peptide cleavage. This design may ensure recognition of endogenous NPC2.
• For Western blotting, anticipate a band at approximately 17 kDa; validate the antibody in positive control lysates (e.g., human fibroblasts or kidney tissue) and consider using a reducing agent given the presence of disulfide bonds.
• In IHC-P or IF-P applications, include appropriate antigen retrieval steps and verify staining on tissue sections known to express NPC2 (such as kidney or testis).
• The antibody cross-reacts with mouse and rat NPC2; however, sequence homology between species should be checked for specific epitopes, and the antibody should be validated in the relevant experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for comprehensive IVD raw-material sourcing, from validated antibody pairs and bulk polyclonal/monoclonal antibodies to ancillary reagents. For NPC2 research—an important target in cholesterol metabolism and Niemann-Pick disease—we offer tailored supply solutions that accelerate your diagnostic or research programs. Whether you need rare-target antibodies or technical consulting, CamelBio bridges the gap from concept to clinical application with reliable, one-stop services.