Anti-NPC1L1 (Niemann-Pick type C1 Like-1) Rabbit Polyclonal Antibody for WB, ELISA - Q9UHC9

Core Product Specifications and Parameters

Biological Background: NPC1-like intracellular cholesterol transporter 1 Function and Localization

• Plays a major role in cholesterol homeostasis and is critical for the uptake of cholesterol across the plasma membrane of intestinal enterocytes. Related references: PMID:22095670
• Direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption and is approved for the treatment of hypercholesterolemia. Related references: PMID:15928087
• May influence lipid metabolism regulation through the transport of multiple lipids and their homeostasis. Related references: PMID:15671032
• Acts as a negative regulator of NPC2, inhibiting its maturation and accelerating its degradation. Related references: PMID:22095670
• Also transports plant sterols such as sitosterol, albeit at lower rates than cholesterol, and may be involved in caveolin trafficking from the plasma membrane.
• Localizes to the apical cell membrane, cell membrane, and cytoplasmic vesicle membrane.
• Widely expressed, with notable expression in liver, small intestine, pancreas, kidney, lung, spleen, heart, gall bladder, brain, testis, stomach, and muscle.
• Contains key structural features including a signal peptide, multiple transmembrane helices, disulfide bonds, glycosylation sites, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 1030-1100). For WB, ensure thorough denaturation of the large 149 kDa protein and consider using 6-8% SDS-PAGE for optimal resolution.
• Due to potential glycosylation, the observed band may migrate slightly above the predicted molecular weight; validate using deglycosylation controls if needed.
• For ELISA, coat the recombinant protein or a peptide spanning the immunogen region and perform checkerboard titrations to optimize antibody and antigen concentrations.
• Given the cross-reactivity with rat, the antibody can be tested in rat intestinal or hepatic tissue lysates; however, titration and validation in the relevant model system are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for cholesterol metabolism and lipid transport research, offering validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. We support the development of diagnostic assays targeting NPC1L1 and related pathways with scalable, research-grade materials. From concept to clinic, CamelBio connects you to hard-to-find raw materials, technical consultancy, and tailored sourcing solutions.