Anti-NPB Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NG41

Core Product Specifications and Parameters

Biological Background: Neuropeptide B Function and Localization

• Neuropeptide B (NPB) is a preproprotein that undergoes proteolytic processing to generate the mature neuropeptide, which belongs to the neuropeptide B/W family.
• It may play roles in the regulation of feeding behavior, neuroendocrine system function, and aspects of memory and learning.
• NPB has been implicated in the afferent pain pathway, suggesting a role in pain perception or modulation.
• The protein is secreted and widely expressed in the central nervous system, with high levels in the substantia nigra, hypothalamus, hippocampus, spinal cord, placenta, and fetal brain.
• Lower expression is detected in testis, uterus, and ovary, and notably, high expression is also found in colorectal adenocarcinoma, indicating potential involvement in cancer.
• The UniProt record classifies NPB under keywords including cleavage on pair of basic residues, direct protein sequencing, and neuropeptide, reflecting its mature peptide processing and functional characterization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-125 of full-length human NPB, which covers the entire preproprotein; therefore, the antibody may recognize both unprocessed and processed forms. Researchers should verify the detected band size in Western blot, which may correspond to the predicted molecular weight of the processed neuropeptide if cleavage occurs in the experimental system.
• For Western blot (WB), consider using reducing conditions and appropriate loading controls from tissues with known NPB expression (e.g., brain regions like hypothalamus). Titrate the primary antibody to achieve optimal signal-to-noise ratio.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization and fixation conditions should be optimized, especially since the target is secreted, to detect intracellular processing intermediates or extracellular signal.
• The antibody is suitable for ELISA; consider its use as a capture or detection reagent in sandwich ELISA after pairing validation. Cross-reactivity with mouse suggests sequence conservation; validate specificity in knockout controls if feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, providing this anti-NPB polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare target raw materials. Whether you are developing diagnostics for neurological disorders or cancer research, we can support your project with custom sourcing and technical consultation, bridging the gap from concept to clinic efficiently.