Anti-NPAS3 Polyclonal Antibody for WB and ELISA - Q8IXF0

Core Product Specifications and Parameters

Biological Background: NPAS3 Function and Localization

• Neuronal PAS domain-containing protein 3 (NPAS3), also known as bHLHe12, MOP6, or PASD6, is a transcription factor encoded by the NPAS3 gene.
• NPAS3 contains basic helix-loop-helix (bHLH) and PAS domains, classifying it as a class E bHLH-PAS family member involved in transcriptional regulation.
• The protein plays a broad role in neurogenesis and may contribute to regulatory pathways associated with schizophrenia and psychotic illness (By similarity).
• NPAS3 localizes to the nucleus, consistent with its DNA-binding and transcription regulatory activities.
• It is ubiquitously expressed in the adult brain, suggesting involvement in diverse neuronal functions.
• The protein undergoes alternative splicing and has been identified in proteomics studies; it is listed as a reference proteome member.
• UniProt keywords: Alternative splicing, Chromosomal rearrangement, DNA-binding, Nucleus, Proteomics identification, Reference proteome, Repeat, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 400–700 of human NPAS3 (based on isoform NP_001158221.1); the antibody may recognize epitopes within this region.
• For Western blot, a band near 101 kDa is expected; validate using human or rat brain tissue lysates as positive controls.
• For ELISA, consider using the recombinant immunogen or NPAS3 protein standards to establish assay conditions.
• Optimise antibody dilution and incubation parameters in your specific experimental system, as reactivity may vary with sample preparation and detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibodies and ancillary reagents for neurobiology research from concept to clinic. Our portfolio includes bulk polyclonal and monoclonal antibodies, antigen pairs, and custom sourcing for rare targets like NPAS3 to support diagnostic assay development. Contact our team to discuss your specific NPAS3 antibody requirements, whether for WB, ELISA, or custom immunoassay platforms.