Anti-NOXA2/p67phox Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P19878

Core Product Specifications and Parameters

Biological Background: NOXA2/p67phox Function and Localization

• NCF2/p67-phox is a key cytosolic subunit of the phagocyte NADPH oxidase complex, which transfers electrons from NADPH to molecular oxygen to generate superoxide, essential for microbial killing. Related references: PMID:12207919 PMID:38355798
• Activation of the oxidase involves phosphorylation-dependent assembly of NCF1/p47-phox with the membrane-bound CYBA/p22-phox, recruiting the cytosolic complex to the nascent phagosome.
• The protein localizes predominantly to the cytoplasm and translocates to membranes upon stimulation.
• Structural domains include an N-terminal TPR repeat and a C-terminal SH3 domain, which mediate protein–protein interactions critical for complex formation.
• Alternative splicing produces multiple isoforms, potentially diversifying regulatory functions.
• Mutations in NCF2 are linked to chronic granulomatous disease (CGD), a primary immunodeficiency characterized by recurrent infections due to defective superoxide production.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 200–300) lies within a central region of p67-phox that is conserved between human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, the predicted molecular weight is ~60 kDa; consider using appropriate positive controls (e.g., neutrophil or macrophage lysates) and validate the signal with appropriate inhibitors or siRNA.
• In IHC-P, antigen retrieval optimization may be required; verify staining specificity on tissues known to express high levels of phagocyte oxidase components, such as spleen or inflamed tissues.
• Due to the monoclonal nature, epitope specificity is consistent, but always confirm reactivity with your target cells under physiological and stimulated conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw-material procurement, connecting you with reliable antibodies, antigen reagents, and ancillary products across diverse research areas. For phagocyte oxidase studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development. Our global sourcing network can also assist with hard-to-find targets, ensuring a streamlined supply chain from concept to clinic.