Anti-NOXA2/p67phox Polyclonal Antibody for WB, IF-P, ELISA - P19878

Core Product Specifications and Parameters

Biological Background: NOXA2/p67phox (NCF2) Function and Localization

• Neutrophil cytosol factor 2 (NCF2), also known as NOXA2, p67-phox, p67-PHOX, and 67 kDa neutrophil oxidase factor, is a cytosolic subunit of the phagocyte NADPH oxidase complex.
• NCF2 is essential for superoxide anion (O2(-)) production by mediating electron transfer from NADPH to O2 within the activated NADPH oxidase complex (PubMed:12207919, PubMed:38355798). Related references: PMID:12207919, PMID:38355798
• Activation of the NADPH oxidase complex is initiated by phosphorylation-dependent binding of the cytosolic NCF1/p47-phox subunit to CYBA/p22-phox, promoting assembly at the plasma membrane or phagosomal membrane.
• The protein localizes to the cytoplasm and translocates to membranes upon activation.
• NCF2 is a phosphoprotein and contains TPR repeats and an SH3 domain, which mediate protein-protein interactions within the oxidase complex.
• Mutations in NCF2 are associated with chronic granulomatous disease, a primary immunodeficiency characterized by defective superoxide production.
• Alternative splicing generates multiple isoforms, and the canonical isoform has a molecular weight of approximately 60 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 227–526 of human NCF2 (NP_000424.2). As a polyclonal antibody, it may recognize multiple epitopes and potentially cross-react with isoforms or orthologs.
• For Western blot (WB), target band expected around 60 kDa; consider optimizing lysate preparation and antibody dilution for clear detection. Validate with appropriate positive and negative controls.
• In immunofluorescence (IF-P), ensure proper fixation and permeabilization to detect cytoplasmic and membrane-translocated NCF2. Use relevant cell lines or primary cells.
• For ELISA, determine optimal coating antigen and antibody titration to achieve desired sensitivity and specificity.
• Cross-reactivity with mouse is reported; validate in your specific sample system if used for non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material partner, offering validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing for diagnostic manufacturers and research laboratories. For projects centered on NADPH oxidase, phagocyte biology, or immunodeficiency research, we can supply matched antibody pairs, optimized polyclonal and monoclonal antibodies, and customized production support. Whether you need concept-to-clinic assistance or reliable supply of critical reagents, CamelBio bridges your sourcing needs with technical expertise.