Anti-NOX2/gp91phox Rabbit Monoclonal Antibody for WB and ELISA - P04839

Core Product Specifications and Parameters

Biological Background: NOX2/gp91phox Function and Localization

• NOX2 (gp91phox), encoded by the CYBB gene, is the catalytic subunit of the phagocyte NADPH oxidase complex, also known as cytochrome b-245 heavy chain.
• It mediates electron transfer from cytosolic NADPH to molecular oxygen, generating superoxide anion (O2•−) essential for host defense. Related references: PMID:15338276, PMID:36241643, PMID:36413210
• Activation of the NADPH oxidase complex requires assembly of cytosolic subunits (e.g., NCF1/p47-phox) with the membrane-bound core complex, triggered by phosphorylation-dependent binding. Related references: PMID:19028840, PMID:38355798
• The protein contains binding sites for FAD, NADP, and two heme moieties, facilitating sequential electron transfer steps for superoxide production.
• Localized to the plasma membrane and phagosomal membrane, enabling directed reactive oxygen species (ROS) generation during phagocytosis.
• Subject to multiple post-translational modifications including phosphorylation, glycosylation, and isopeptide bond formation, which may regulate activity and trafficking.
• Mutations in CYBB cause chronic granulomatous disease (CGD), an immunodeficiency characterized by defective superoxide production.
• Detected predominantly in neutrophils at the protein level, highlighting its role in innate immune responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 of human NOX2/gp91phox. This region may encompass extracellular or membrane-proximal domains, suggesting the antibody could recognize both native and denatured protein.
• For Western blotting, a band around 65 kDa is expected under reducing conditions; validate the signal in relevant human, mouse, or rat neutrophil lysates.
• When using ELISA, consider coating with the immunogen peptide or recombinant NOX2 protein to confirm specificity.
• Since NOX2 activation involves complex assembly, the antibody may be useful for pull-down assays to study protein-protein interactions, but such applications should be individually optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. We specialize in sourcing rare-target antibodies like anti-NOX2/gp91phox to support phagocyte biology and immunodeficiency research. Contact us for technical consulting and seamless procurement from concept to clinic.