Anti-NOX2/gp91phox Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04839

Core Product Specifications and Parameters

Biological Background: NOX2/gp91phox Function and Localization

• NADPH oxidase 2 (NOX2), also known as gp91phox, is the catalytic subunit of the phagocyte NADPH oxidase complex, a critical enzyme in innate immunity.
• Catalyzes the transfer of electrons from cytosolic NADPH to molecular oxygen to produce superoxide anion, a reactive oxygen species essential for microbial killing. Related references: PMID:15338276 PMID:36241643 PMID:36413210
• Electron transfer proceeds via flavin adenine dinucleotide (FAD) and two heme molecules, culminating in an outer-sphere reaction that generates superoxide. Related references: PMID:38355798
• Activation involves phosphorylation-dependent binding of cytosolic NCF1/p47-phox to the C-terminus of CYBA/p22-phox, leading to assembly of the active complex at the plasma membrane or phagosomal membrane. Related references: PMID:19028840 PMID:38355798
• Localizes primarily to the plasma membrane and phagosomal membranes, positioning it for spatial control of reactive oxygen species production.
• Expressed predominantly in neutrophils, consistent with its role in the professional phagocyte oxidative burst.
• Mutations in the CYBB gene encoding NOX2 are associated with X-linked chronic granulomatous disease, an immunodeficiency characterized by impaired respiratory burst and recurrent infections.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region of NOX2 (aa 385–570), suggesting the antibody may recognize both denatured and native conformations; consider its suitability for WB and immunofluorescence based on your sample preparation.
• For WB, the predicted molecular weight is ~65 kDa for the precursor; the mature glycoprotein can migrate at ~91 kDa. Validate the observed band in your specific lysate and include appropriate controls.
• In IHC-P and IF/ICC, expect membrane and phagosomal staining. Adequate permeabilization is required for intracellular epitope access; optimize fixation and permeabilization conditions.
• For ELISA, peptide or recombinant protein coating may be used to capture the antibody. Consider coating buffer, blocking conditions, and antibody titration to establish optimal signal-to-noise.
• Cross-reactivity with mouse and rat suggests epitope conservation; validate specificity in these species by knockout tissue or peptide competition.
• Functional studies may involve stimulation of phagocytes (e.g., with PMA) to observe translocation or enhanced detection; ensure appropriate controls for activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated supply solutions for IVD developers, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our NOX2/gp91phox antibody supports research into phagocyte oxidative burst mechanisms and chronic granulomatous disease biomarker development. We also assist in sourcing rare-target raw materials and provide technical consulting to streamline your assay development from concept to clinic.