Anti-NOX2/gp91phox Polyclonal Antibody for WB, ELISA - P04839

Core Product Specifications and Parameters

Biological Background: NOX2/gp91phox Function and Localization

• NOX2/gp91phox, also known as CYBB or NADPH oxidase 2, is the heavy chain catalytic subunit of the phagocyte cytochrome b-245 complex.
• Catalytic subunit mediating electron transfer from cytosolic NADPH to O2 to produce superoxide anion, a precursor to reactive oxygen species critical for microbial killing (PubMed:15338276, PubMed:36241643, PubMed:36413210). Related references: PMID:15338276 PMID:36241643 PMID:36413210
• Electron transfer involves sequential reduction of FAD and two heme cofactors to reduce O2 (PubMed:38355798). Related references: PMID:38355798
• Activation requires assembly of cytosolic subunits (e.g., p47-phox/NCF1) with the membrane-bound cytochrome b-245, triggered by phosphorylation and membrane translocation (PubMed:19028840, PubMed:38355798). Related references: PMID:19028840 PMID:38355798
• Localized primarily at the cell membrane and phagosomal membrane of phagocytes.
• Detected in neutrophils, where it plays a central role in the respiratory burst.
• Mutations in CYBB cause X-linked chronic granulomatous disease (CGD), characterized by defective superoxide production and recurrent infections.
• Contains FAD- and NADPH-binding domains, two hemes, and is N-glycosylated; regulated by phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to human NOX2/gp91phox amino acids 101-200; the resulting polyclonal antibody may recognize multiple epitopes within this region. It is recommended to validate specificity using positive (e.g., neutrophil lysates) and negative controls.
• For Western blotting, a primary band around 65 kDa is expected for the unglycosylated form; additional bands at higher molecular weights may correspond to glycosylated species (gp91phox). Optimize blocking and detection conditions accordingly.
• The antibody is reported to cross-react with mouse and rat; however, due to sequence variations, confirm reactivity in your specific model system.
• In ELISA applications, consider peptide competition assays to assess specificity.
• Since the target is a transmembrane protein, inclusion of mild detergents in lysis buffers may improve solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including validated antibodies and recombinant proteins for NADPH oxidase and redox biology research. This anti-NOX2/gp91phox polyclonal antibody, along with our extensive portfolio of ancillary reagents, supports diagnostic development for chronic granulomatous disease and immune function assays. Partner with us for reliable, scalable sourcing from discovery through clinical diagnostics.