Anti-NOX1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5S8

Core Product Specifications and Parameters

Biological Background: NOX1 Function and Localization

• NOX1, also known as MOX1 (mitogenic oxidase 1), NOH-1, or GP91-2, is a member of the NADPH oxidase family of enzymes that generate reactive oxygen species (ROS).
• The primary function of NOX1 is to transfer electrons from NADPH to molecular oxygen, generating superoxide anions. This activity requires cofactors including FAD, heme, and iron.
• The NOX1 gene is predominantly expressed in the colon, uterus, and prostate, and has been detected in colon carcinoma cells, implicating its role in cell proliferation and cancer biology.
• NOX1 localizes to the cell membrane and invadopodium membrane, a specialized cell projection involved in extracellular matrix degradation, linking its ROS production to cell invasion and migration.
• As indicated by its alternative name "mitogenic oxidase 1", NOX1-mediated superoxide production can promote mitogenic signaling pathways, potentially contributing to tumorigenesis in colon and prostate cancers.
• Post-translational modifications of NOX1 include phosphorylation and glycosylation, which may regulate its activity and localization.
• The human NOX1 gene produces multiple alternatively spliced transcript variants, which may contribute to the functional diversity and regulation of NOX1 activity in different tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region of human NOX1 (aa 200-300). Consider this when assessing isoform specificity or if using the antibody for detection of alternatively spliced variants.
• For Western blotting, the predicted molecular weight is approximately 65 kDa; however, glycosylation may cause an upward shift. Validate the antibody in the target tissue or cell line.
• In immunofluorescence (IF/ICC), expect membrane and invadopodium staining. Optimize fixation and permeabilization to preserve membrane structures.
• For ELISA, use the immunogen peptide as a positive control to evaluate antibody titer and specificity.
• Cross-reactivity has been confirmed only for human. Validate in other species if needed before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. The NOX1 antibody enriches our cancer and cell signaling portfolio; contact us for custom sourcing of rare targets and tailored technical support.