Anti-NOX1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y5S8

Core Product Specifications and Parameters

Biological Background: NOX1 Function and Localization

• NADPH oxidase 1 (NOX1), also known as mitogenic oxidase 1 (MOX-1), NOH-1, and GP91-2, is a member of the NOX family of enzymes that generate reactive oxygen species.
• Catalyzes the generation of superoxide from molecular oxygen using NADPH as an electron donor, contributing to cellular signaling, host defense, and proliferative responses.
• Localizes to the cell membrane and invadopodium membrane, positioning it at sites of cell adhesion and matrix degradation.
• Expressed in colon, uterus, prostate, and colon carcinoma, but absent from peripheral blood leukocytes, highlighting tissue-specific roles in epithelial cells and cancer.
• Contains FAD and heme cofactors essential for electron transport, and features multiple transmembrane helices.
• Undergoes alternative splicing and phosphorylation, indicating post-translational regulation of its activity and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 241–340 of human NOX1, a region predicted to be unique to NOX1; verify specificity using NOX1 knockout or overexpression systems.
• In Western blot (WB), a band around 65 kDa is expected in human colon or prostate tissue lysates; use a molecular weight marker and appropriate loading controls.
• For ELISA applications, consider coating with the immunogen peptide as a positive control to optimize assay conditions.
• Non-specific binding can be minimized by titrating the antibody and using appropriate blocking agents; validate the antibody in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-NOX1 monoclonal antibody is a valuable tool for studying oxidative stress, colorectal cancer, and inflammatory diseases. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and custom sourcing services to meet your entire assay development needs.