Anti-NOX1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y5S8

Core Product Specifications and Parameters

Biological Background: NOX1 Function and Localization

• NOX1 (NADPH oxidase 1), also designated mitogenic oxidase 1 (MOX-1) or NOH-1, is a transmembrane enzyme belonging to the NADPH oxidase family.
• It functions as an electron transporter, catalyzing the generation of superoxide (O2-) from molecular oxygen using NADPH as the electron donor.
• The enzyme contains FAD and heme-binding domains, characteristic of flavoproteins and metalloproteins involved in redox reactions.
• NOX1 localizes predominantly to the cell membrane and is also enriched at invadopodium membranes, specialized cell projections involved in matrix degradation.
• Expression is noted in colon, uterus, and prostate tissues, as well as in colon carcinoma, but it is absent from peripheral blood leukocytes.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing, contributing to functional diversity.
• Associated keywords: Alternative splicing, Cell junction, Electron transport, FAD, Flavoprotein, Glycoprotein, Heme, Iron, Membrane, Metal-binding, NADP, Oxidoreductase, Phosphoprotein, Transmembrane, Transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 418-564 of human NOX1, targeting the C-terminal cytosolic region. This design may allow detection of total NOX1 protein.
• For Western blotting (WB), consider using whole-cell lysates from colon carcinoma cell lines (e.g., HCT 116) or mouse colon tissue as positive controls.
• For immunohistochemistry (IHC-P), optimal antigen retrieval and antibody dilution should be validated in the target tissue of interest, such as human colon sections.
• ELISA performance may require coating with the immunogen peptide or recombinant protein; confirm linearity and specificity with serial dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and research labs with on-demand access to validated antibodies and ancillary reagents. With expertise in the NOX/NADPH oxidase family, we can assist in sourcing rare-target antibodies, matched antibody pairs, and bulk polyclonal antibodies to accelerate your assay development. Whether you require the NOX1 Rabbit pAb for oxidative stress studies or need a full suite of companion raw materials, CamelBio bridges your concept-to-clinic journey with reliable supply and technical consultation.