Anti-NOX1 Polyclonal Antibody for WB, ELISA - Q9Y5S8

Core Product Specifications and Parameters

Biological Background: NADPH Oxidase 1 Function and Localization

• NADPH oxidase 1 (NOX1), also known as mitogenic oxidase 1 (MOX-1) or NOH-1, is a member of the NOX family of NADPH oxidases that generate reactive oxygen species (ROS) as their primary function.
• NOX1 catalyzes the electron transfer from NADPH to molecular oxygen to produce superoxide, a precursor for various ROS that participate in host defense, cell proliferation, and signal transduction.
• The enzyme is a multi-pass transmembrane glycoprotein containing binding sites for FAD, NADPH, and two heme groups, and is regulated by assembly with cytosolic subunits.
• Structurally related to the phagocyte NADPH oxidase gp91-phox (NOX2), NOX1 is predominantly expressed in colon epithelium, with additional expression in uterus, prostate, and certain carcinomas, but not in peripheral blood leukocytes.
• Alternative splicing generates multiple isoforms that may differ in tissue distribution and regulatory properties.
• NOX1 localizes to the plasma membrane and specialized membrane invadopodia, where local ROS production promotes cell invasion and migration.
• Post-translational modifications include glycosylation and phosphorylation, which may modulate activity and protein interactions.
• Keywords: Alternative splicing, Cell membrane, Electron transport, FAD, Flavoprotein, Glycoprotein, Heme, Iron, Membrane, Metal-binding, NADP, Oxidoreductase, Phosphoprotein, Transmembrane, Transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 241–340, located in the intracellular region of NOX1; this region is predicted to be conserved and suitable for rabbit polyclonal antibody production.
• For Western blotting, consider using whole-cell lysates from colon or colon carcinoma cell lines (e.g., Caco-2, HT-29) where NOX1 is endogenously expressed; a band near 65 kDa is expected.
• When performing ELISA, validate the antibody in direct or capture formats against recombinant fragments or full-length NOX1, as the polyclonal nature may require optimization.
• Take into account potential cross-reactivity with other NOX family members due to conserved domains; verify specificity using knockout controls or isoform-specific blocking peptides.
• For immunohistochemistry or immunofluorescence, preliminary testing is advised as the antibody’s performance in these applications is not pre-validated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw material partner, CamelBio supports diagnostic developers and research labs in accessing high-quality antibodies, antigens, and ancillary reagents for assay development and biomarker studies. This NOX1 polyclonal antibody fits within oxidative stress, inflammation, and cancer-related research programs, and CamelBio can facilitate bulk sourcing, matched antibody pairs, and tailored manufacturing support. For rare or custom targets like NADPH oxidase proteins, our one-stop supply chain simplifies procurement from early-stage R&D to validated IVD kits.