Anti-NOTCH4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99466

Core Product Specifications and Parameters

Biological Background: NOTCH4 Function and Localization

• NOTCH4 (Neurogenic locus notch homolog protein 4) is a member of the Notch family of transmembrane receptors, which are key regulators of cell-fate determination.
• It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1, mediating short-range cell-cell communication.
• Upon ligand binding, the intracellular domain (NICD) is cleaved and translocates to the nucleus, where it forms a transcriptional activator complex with RBPJ/RBPSUH to activate target genes.
• NOTCH4 signaling modulates diverse cellular processes including differentiation, proliferation, and apoptosis, with a potential role in vascular branching morphogenesis (by similarity).
• The protein is localized at the cell membrane and, upon activation, in the nucleus.
• NOTCH4 expression is tissue-specific: highly expressed in heart, moderately in lung and placenta, and at low levels in several other tissues; it is absent from adult brain and peripheral blood leukocytes.
• Structurally, NOTCH4 contains multiple EGF-like repeats, ANK repeats, and other domains typical of Notch receptors; it undergoes post-translational modifications including glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning the C-terminal region (aa 1824-2003) of human NOTCH4, which may detect both full-length and cleaved intracellular domain.
• For Western blotting, a band of ~210 kDa corresponding to full-length NOTCH4 may be observed; however, due to proteolytic processing, additional lower molecular weight bands might appear. Validation in the relevant sample system is recommended.
• In immunocytochemistry/immunofluorescence (IF/ICC), anticipate membrane localization in unstimulated cells and both membrane and nuclear staining following ligand activation.
• The antibody is also suitable for ELISA applications for detection or capture of NOTCH4.
• Cross-reactivity with mouse and rat has been reported, but performance should be verified under specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, technical services, and specialist consulting—supporting manufacturers, laboratories, and research institutes from concept to clinic. This NOTCH4 antibody aligns with our portfolio of Notch signaling and developmental biology reagents. For your IVD development, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary materials, and access to rare-target sourcing capabilities. Contact our team to explore custom supply solutions.