Anti-NOTCH3 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UM47

Core Product Specifications and Parameters

Biological Background: NOTCH3 Function and Localization

• NOTCH3 is a member of the Notch family of transmembrane receptors, encoded by the NOTCH3 gene, and is listed under aliases including IMF2, LMNS, CASIL, and CADASIL1.
• It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Related references: PMID:15350543 PMID:14714274
• Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, activating genes of the enhancer of split locus and thereby affecting differentiation, proliferation, and apoptotic programs.
• Subcellular localization includes the cell membrane and nucleus, consistent with its receptor function and nuclear signaling role.
• It is ubiquitously expressed in fetal and adult tissues, indicating widespread regulatory roles.
• The protein contains structural features such as EGF-like domains, ANK repeats, and a transmembrane helix, and is subject to post-translational modifications including glycosylation and phosphorylation.
• Mutations in NOTCH3 are associated with CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy), highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular region (aa 2200-2321); the antibody may detect both full-length receptor (~244 kDa) and the cleaved NICD fragment.
• For Western blotting, the high molecular weight (~244 kDa) requires prolonged transfer times and appropriate gel percentages; include positive controls from human, mouse, or rat lysates.
• In IHC-P, antigen retrieval conditions may influence staining; validate in tissue sections known to express NOTCH3.
• ELISA performance should be confirmed using recombinant NOTCH3 protein or peptide as coating antigen.
• Consider titrating the antibody for each application to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated IVD raw material solutions, offering validated antibodies, bulk reagents, and rare-target sourcing. This NOTCH3 polyclonal antibody supports research into Notch signaling, developmental biology, and CADASIL mechanisms, and can be incorporated into custom assay development. With an extensive portfolio and technical expertise, CamelBio streamlines your transition from concept to clinic.