Anti-NOTCH3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UM47

Core Product Specifications and Parameters

Biological Background: NOTCH3 Function and Localization

• NOTCH3 is a member of the Notch family of single-pass transmembrane receptors that regulate cell-fate determination through the Notch signaling pathway.
• Functions as a receptor for membrane-bound ligands Jagged1 (JAG1), Jagged2 (JAG2), and Delta-like 1 (DLL1), initiating signal transduction upon ligand binding. Related references: PMID:15350543 PMID:14714274
• Ligand activation triggers cleavage of the intracellular domain (NICD), which translocates to the nucleus and forms a transcriptional activator complex with RBPJ/RBPSUH to activate downstream target genes.
• NOTCH3 influences key cellular processes including differentiation, proliferation, and apoptosis, playing critical roles in development and tissue homeostasis.
• It localizes to the cell membrane and, upon activation, to the nucleus, consistent with its dual function in signal reception and transcription regulation.
• The protein is ubiquitously expressed in both fetal and adult tissues, underscoring its broad regulatory roles.
• NOTCH3 contains multiple EGF-like repeat domains and ANK repeats, and is subject to post-translational modifications such as glycosylation, phosphorylation, and methylation.
• Mutations in NOTCH3 are associated with CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy), a hereditary stroke disorder.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a band at approximately 244kDa; using a low-percentage gel (e.g., 6–8% acrylamide) and extended transfer times is recommended for high-molecular-weight proteins.
• In immunocytochemistry/immunofluorescence (IF/ICC), optimize permeabilization conditions to detect both membrane and nuclear staining patterns.
• The antibody’s cross-reactivity with mouse and rat NOTCH3 suggests potential utility in comparative studies; validation in the target species and experimental system is advised.
• For ELISA applications, consider the polyclonal nature and potential for broader epitope recognition when designing capture/detection assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with a comprehensive selection of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For studies focused on Notch signaling or CADASIL, we supply high-quality NOTCH3 antibodies and related proteins, helping streamline your workflow from discovery to clinical application. Our team can also assist with sourcing rare-target raw materials and custom solutions to meet your specific project needs.