Anti-NOTCH2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q04721

Core Product Specifications and Parameters

Biological Background: NOTCH2 Function and Localization

• Neurogenic locus notch homolog protein 2 (NOTCH2) is a receptor in the Notch signaling pathway, critical for cell-fate determination, differentiation, proliferation, and apoptotic programs (By similarity).
• NOTCH2 is activated by membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2), and Delta-1 (DLL1), leading to release of the Notch intracellular domain (NICD) that forms a transcriptional activator complex with RBPJ/RBPSUH to regulate target genes (PubMed:21378985, PubMed:21378989). Related references: PMID:21378985, PMID:21378989
• It plays a role in bone remodeling and homeostasis, positively regulating RANKL-induced osteoclast differentiation through collaboration with RELA/p65 to enhance NFATc1 promoter activity (PubMed:29149593). Related references: PMID:29149593
• NOTCH2 is involved in liver cancer cell self-renewal (PubMed:25985737). Related references: PMID:25985737
• Subcellular localization includes the cell membrane, nucleus, and cytoplasm.
• Tissue expression is detected in brain, heart, kidney, lung, skeletal muscle, and liver, and it is ubiquitously expressed in the embryo.
• NOTCH2 contains EGF-like domains, ANK repeats, and a transmembrane helix; it undergoes post-translational modifications such as phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• For western blotting (WB), consider using a low-percentage SDS-PAGE gel (e.g., 6%) to resolve the high-molecular-weight target (~265 kDa) and validate the band in a suitable cell or tissue lysate.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions, as NOTCH2 can localize to multiple subcellular compartments.
• In ELISA, titrate the antibody in a direct or capture format and evaluate performance against a standard curve of the immunogen or a relevant NOTCH2 fragment.
• As a polyclonal antibody, lot-to-lot variability may exist; include appropriate controls and consider pre-adsorption if specificity issues arise.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with comprehensive, one-stop access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For projects targeting NOTCH2—whether investigating Notch signaling, bone remodeling, liver cancer, or developmental biology—we offer customized procurement of monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services to streamline your assay development from concept to clinic.