Anti-Notch1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46531

Core Product Specifications and Parameters

Biological Background: Notch1 Function and Localization

• Notch1 functions as a receptor for membrane-bound ligands Jagged-1, Jagged-2, and Delta-1, regulating cell-fate determination through the canonical Notch signaling pathway.
• Upon ligand activation, the Notch intracellular domain (NICD) is released and translocates to the nucleus to form a transcriptional activator complex with RBPJ/RBPSUH, driving expression of enhancer of split locus genes.
• Affects the implementation of differentiation, proliferation, and apoptotic programs; negatively regulates endothelial cell proliferation and migration, thereby contributing to angiogenesis control.
• Important for the maturation of both CD4+ and CD8+ cells in the thymus and for follicular differentiation, possibly influencing cell fate selection within the follicle.
• During cerebellar development, acts as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.
• Represses neuronal and myogenic differentiation; may play an essential role in postimplantation development, mesoderm development, somite formation, and neurogenesis.
• Localizes to the cell membrane, late endosome membrane, and nucleus (after cleavage and nuclear translocation of NICD).
• In fetal tissues, most abundant in spleen, brain stem, and lung; in adult tissues, found mainly in lymphoid tissues.

Experimental Guidance and Technical Tips

• When using this antibody for western blot, consider that the full-length Notch1 has a predicted molecular weight of approximately 273 kDa, but cleavage products may also be observed. Validate the band pattern in your specific sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and fixation conditions may influence staining. Optimize protocols using appropriate positive and negative controls.
• The immunogen corresponds to amino acids 2428–2555 in the C-terminal region of human Notch1; the antibody may recognize both full-length and cleaved forms depending on sample preparation.
• Cross-reactivity with mouse and rat Notch1 is indicated; however, we recommend verifying reactivity and specificity in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials. This Notch1 polyclonal antibody supports studies in Notch signaling, development, and disease and exemplifies our portfolio of validated antibodies. From optimized antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio bridges the gap from concept to clinic with reliable raw-material solutions.