Anti-NOSTRIN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IVI9

Core Product Specifications and Parameters

Biological Background: NOSTRIN Function and Localization

• NOSTRIN (Nitric Oxide Synthase TRAffic INducer) is a multivalent adapter protein that negatively regulates endothelial nitric oxide synthase (eNOS/NOS3) by promoting its translocation from the plasma membrane to intracellular compartments.
• Alternative names include BM247 homolog, nitric oxide synthase traffic inducer, nitric oxide synthase trafficker, and eNOS-trafficking inducer, reflecting its role in NOS3 trafficking.
• The protein localizes to multiple subcellular sites, including the cell membrane, cytoplasmic vesicles, cytoplasm, cytoskeleton, and nucleus, indicating dynamic trafficking and diverse functional roles.
• Highest expression levels are found in heart, kidney, placenta, and lung, with lower levels in brain, thymus, and spleen. It is present in vascular endothelial cells and placenta, and is overexpressed in placental tissue from pre-eclamptic pregnancies.
• Structural features include an SH3 domain and coiled-coil regions that mediate protein-protein interactions essential for its adapter function. The protein undergoes alternative splicing and phosphorylation.
• Functional keywords highlight involvement in endocytosis and membrane trafficking, consistent with its role in relocalizing eNOS.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270-430 of human NOSTRIN (isoform 1). When performing western blot, consider that the predicted molecular weight is approximately 57.7 kDa, with detection expected around 58 kDa.
• For immunocytochemistry/immunofluorescence, subcellular localization patterns may include membrane, vesicular, and cytoskeletal staining. Validate fixation and permeabilization conditions for your cell type.
• ELISA applications may benefit from pairing this antibody with a suitable detection antibody; consider the polyclonal nature when establishing sandwich assays.
• Cross-reactivity with mouse and rat orthologs has been observed; however, we recommend verifying sequence homology in the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This NOSTRIN antibody supports research into eNOS trafficking and vascular biology, and we can assist with matching detection reagents and custom assay development. Let CamelBio streamline your transition from concept to clinic with reliable, one-stop access to critical raw materials.