Anti-NOS1 Polyclonal Antibody for WB & ELISA - P29475

Core Product Specifications and Parameters

Biological Background: NOS1 Function and Localization

• NOS1, or nitric oxide synthase 1, is also known as neuronal NOS (nNOS), bNOS, NC-NOS, and NOS type I. It is a constitutive NOS isoform that produces nitric oxide (NO), a versatile messenger molecule with neurotransmitter-like properties in the brain and peripheral nervous system.
• The enzyme likely possesses nitrosylase activity, mediating cysteine S-nitrosylation of cytoplasmic target proteins such as SRR, thereby modulating protein function through post-translational modification.
• NOS1 is a multi-domain oxidoreductase that binds calmodulin, FAD, FMN, heme (iron), and NADP, and is subject to phosphorylation and ubiquitination.
• Encoding multiple isoforms via alternative splicing, isoform 1 shows ubiquitous expression (skeletal muscle, brain, testis, lung, kidney; low in heart, adrenal, retina). Isoform 3 is testis-specific, while isoform 4 is detected in testis, skeletal muscle, lung, kidney, and at low levels in brain.
• At the subcellular level, NOS1 localizes to the cell membrane, sarcolemma, dendritic spines, and synapses, consistent with its role in neuronal signaling.
• As a flavoprotein and heme-containing enzyme, NOS1 catalyzes the NADPH-dependent oxidation of L-arginine to NO and L-citrulline, playing a critical role in vasodilation, neurotransmission, and immune response.
• The protein has a molecular weight of approximately 161 kDa (UniProt: 160970 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–180) of human NOS1. Consider this when selecting detection epitopes in WB or designing competitive ELISA setups.
• For Western blot, expect a band near 161 kDa; validate specificity in your sample system, especially when working with tissue lysates from human or rat.
• Cross-reactivity with rat enables potential use in rat neuronal, muscle, or testis samples, but experimental verification is recommended due to possible isoform-specific differences.
• ELISA performance should be optimized with the appropriate antigen coating and detection reagents; consider using recombinant NOS1 protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

NOS1 is central to nitric oxide signaling in neuroscience and cardiovascular research. CamelBio supports diagnostic manufacturers and research labs by offering one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-NOS1 product, bulk ancillary reagents, and custom sourcing for rare targets. Our portfolio streamlines your assay development from concept to clinic.