Anti-NOP58 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2X3

Core Product Specifications and Parameters

Biological Background: NOP58 Function and Localization

• NOP58 (Nucleolar protein 58), also known as NOP5/NOL5, is a conserved nucleolar protein essential for ribosome biogenesis.
• It is required for the biogenesis of box C/D small nucleolar RNAs (snoRNAs) such as U3, U8, and U14, which guide site-specific 2'-O-methylation of ribosomal and messenger RNAs. Related references: PMID:15574333 PMID:17636026 PMID:19620283
• NOP58 is a core component of box C/D snoRNP complexes that catalyze RNA methylation, a modification critical for ribosome assembly and function. Related references: PMID:39570315
• It participates in the assembly of the small subunit (SSU) processome, the earliest precursor of the small ribosomal subunit, coordinating RNA folding, cleavage, and targeted degradation. Related references: PMID:34516797
• The protein localizes to the nucleolus and nucleoplasm, consistent with its roles in pre-rRNA processing and snoRNP maturation.
• Ubiquitously expressed across human tissues, reflecting the fundamental requirement for ribosome production in all cell types.
• Post‑translational modifications include phosphorylation and ubiquitination, and a solved 3D‑structure provides insights into snoRNA association.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 360–529 of human NOP58, a region likely conserved across species, supporting the observed cross‑reactivity with mouse and rat.
• For western blotting, a band near 60 kDa is expected; consider using appropriate molecular weight markers and validating loading controls.
• Immunofluorescence/immunocytochemistry should reveal nuclear enrichment with nucleolar puncta; co‑staining with nucleolar markers may confirm specificity.
• ELISA can be used for quantitative detection, but assay conditions must be optimized within individual experimental systems.
• As with all polyclonal antibodies, lot‑to‑lot variability may occur; validate each new lot in the relevant sample system before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high‑quality IVD raw materials. This anti‑NOP58 antibody supports investigations into ribosome biogenesis, nucleolar biology, and snoRNP research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare‑target raw materials to accelerate your project from concept to clinic.