Anti-NOP56 Polyclonal Antibody for WB, ELISA - O00567

Core Product Specifications and Parameters

Biological Background: NOP56 Function and Localization

• NOP56 (Nucleolar protein 56), also known as NOL5A or SCA36, is a core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that mediate site-specific 2'-O-methylation of ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs). Related references: PMID:12777385 PMID:39570315
• It plays a central role in the early to middle stages of 60S ribosomal subunit biogenesis and is essential for the biogenesis of box C/D snoRNAs such as U3, U8, and U14. Related references: PMID:12777385 PMID:15574333
• NOP56 is an integral part of the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit, where it contributes to pre-rRNA folding, modification, and processing. Related references: PMID:34516797
• The protein localizes primarily to the nucleolus and nucleoplasm, with additional presence in the cytoplasm, reflecting its dynamic role in ribosome assembly.
• NOP56 undergoes various post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond formation), which may regulate its function and interactions.
• Mutations in the NOP56 gene are associated with spinocerebellar ataxia type 36 (SCA36), a neurodegenerative disorder, highlighting its clinical relevance beyond basic cellular processes.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-160) of human NOP56, a conserved domain critical for protein interactions—validate specificity in your sample system using a suitable control lysate or blocking peptide.
• For Western blot detection, the predicted molecular weight is 66 kDa; ensure proper gel percentage and transfer conditions, and consider that post-translational modifications may cause slight band shifts.
• This antibody is recommended for ELISA; for quantitative applications, titrate the antibody and confirm performance with recombinant NOP56 protein or known-positive cell extracts.
• Cross-reactivity is reported for human; if working with other species, perform sequence alignment of the immunogen region to assess potential reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, supporting the entire development pipeline from concept to clinic. In the field of ribosome biology and neurodegeneration, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for targets like NOP56. Our sourcing capabilities also extend to rare targets and customized reagent development, helping you accelerate assay development and biomarker discovery.