Anti-NOP2 Polyclonal Antibody for WB, ELISA - P46087

Core Product Specifications and Parameters

Biological Background: NOP2 Function and Localization

• NOP2, also known as NOL1, NSUN1, or p120, is a proliferation-associated nucleolar antigen and 28S rRNA (cytosine(4447)-C(5))-methyltransferase.
• It specifically catalyzes the S-adenosyl-L-methionine-dependent methylation of cytosine 4447 in 28S rRNA, a critical modification for ribosomal RNA maturation (PubMed:26196125).
  Related references:
  PMID:26196125
• The enzyme is essential for efficient pre-rRNA processing and 60S ribosomal subunit biogenesis (PubMed:24120868, PubMed:36161484).
  Related references:
  PMID:24120868, PMID:36161484
• Through non-catalytic interactions with box C/D snoRNAs, NOP2 facilitates the stable assembly of U3 and U8 snoRNPs into pre-90S ribosome particles (PubMed:36161484).
  Related references:
  PMID:36161484
• The protein resides in the nucleolus and nucleoplasm and may contribute to cell cycle regulation and the elevated nucleolar activity characteristic of proliferating cells (PubMed:24120868).
  Related references:
  PMID:24120868
• NOP2 undergoes multiple post-translational modifications, including acetylation, citrullination, phosphorylation, and isopeptide bond formation, suggesting complex regulatory mechanisms.
• Alternative splicing of the NOP2 pre-mRNA generates isoforms, and the protein is a target for ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The recombinant immunogen spanning amino acids 355–590 of human NOP2 covers a region that may be important for functional studies; consider this when designing epitope-based assays.
• For Western blot (WB), a band around 89 kDa (full-length) is expected; validate the antibody in the relevant species (human, mouse) using appropriate positive controls.
• In ELISA, pre-adsorption and titration experiments are recommended to optimize signal-to-noise and determine working dilutions.
• Cross-reactivity with mouse NOP2 has been confirmed by the supplier, but additional verification with your specific mouse tissue or cell line is advisable.
• This polyclonal antibody may detect multiple bands due to post-translational modifications or isoforms; include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a premier one-stop bridge for IVD raw materials, offering validated polyclonal antibodies like anti-NOP2 that support research into ribosomal biogenesis and proliferative diseases. With a comprehensive portfolio of antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents, CamelBio enables diagnostic manufacturers and research labs to efficiently source the components they need, from discovery through clinical application.