Anti-NOP16 Polyclonal Antibody for WB, ELISA - Q9Y3C1

Core Product Specifications and Parameters

Biological Background: NOP16 Function and Localization

• Nucleolar protein 16 (NOP16) is also known as HSPC111, HSPC185, and HBV pre-S2 trans-regulated protein 3.
• The protein localizes to the nucleus, specifically the nucleolus, suggesting a role in ribosome biogenesis or other nucleolar functions.
• NOP16 undergoes alternative splicing, yielding multiple isoforms with potentially distinct activities or subcellular distributions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (e.g., isopeptide bond formation), which may regulate protein stability or interactions.
• The protein has been identified in large-scale proteomics studies and is part of the human reference proteome.
• Three-dimensional structural data (PDB) are available, providing a basis for molecular interaction studies.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the full-length human NOP16 (aa 1-178); consider using the recombinant protein as a positive control in downstream assays.
• For Western blot, the predicted molecular weight is approximately 21 kDa; note that alternative splicing or post-translational modifications may lead to additional bands.
• In ELISA applications, titrate the antibody and coat with the immunogen or native protein to optimize signal-to-noise ratio.
• Since this is a polyclonal antibody, lot-to-lot variability is possible; validate each new lot in the relevant sample system.
• Cross-reactivity with mouse and rat NOP16 has been documented; confirm reactivity in your specific tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, from early-stage R&D to clinical applications. For NOP16-related research, we can support your projects with validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and procurement of rare or custom targets. Our one-stop approach streamlines the path from concept to clinic, ensuring reliable supply and technical support for diagnostic manufacturers and laboratories.