Anti-NOP16 Polyclonal Antibody for WB, ELISA - Q9Y3C1

Core Product Specifications and Parameters

Biological Background: NOP16 Function and Localization

• Nucleolar protein 16 (NOP16) is a nucleus-localized protein predominantly found in the nucleolus, as annotated in UniProt.
• NOP16 is also known as HBV pre-S2 trans-regulated protein 3, suggesting a potential link to viral protein interactions, though its primary role is nucleolar.
• The protein undergoes alternative splicing, generating multiple isoforms that may exhibit distinct functional or regulatory properties.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, indicating dynamic regulation.
• A 3D structure has been determined for NOP16, providing insights into its molecular architecture and potential interaction surfaces.
• NOP16 has been identified through proteomics studies, confirming its expression and modification status in human cells.
• Its presence as a reference proteome component underscores its evolutionary conservation and fundamental cellular significance.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the full-length human NOP16 (residues 1–178), which may enable recognition of multiple isoforms due to the conserved N-terminal region.
• For Western blotting, a band at approximately 21 kDa is expected; however, observe for possible isoform-specific variations or post-translational modification-induced shifts.
• In ELISA applications, ensure coating conditions and antibody titrations are optimized for the specific assay format; cross-reactivity with mouse NOP16 suggests utility in rodent models.
• Since NOP16 localizes to the nucleolus, consider subcellular fractionation to enrich the target protein for more sensitive detection in complex samples.
• Validate antibody specificity by including appropriate controls, such as knockdown or knockout lysates, in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to streamline IVD raw-material procurement from concept to clinic. This anti-NOP16 polyclonal antibody supports nucleolar biology investigations and ribosome biogenesis research, and CamelBio can also supply validated antibody pairs, optimized monoclonal alternatives, bulk ancillary reagents, or source rare targets on request. Let us bridge your supply needs so you can focus on breakthrough diagnostics.