Anti-NOP14 Polyclonal Antibody for WB, ELISA - P78316

Core Product Specifications and Parameters

Biological Background: NOP14 Function and Localization

• Nucleolar protein 14 (NOP14), also known as UTP2, NOL14, C4orf9, RES425, or RES4-25, is a conserved nucleolar protein involved in ribosome biogenesis.
• NOP14 participates in the nucleolar processing of pre-18S ribosomal RNA, an essential step in the maturation of the small ribosomal subunit.
• It is also implicated in the nuclear export of the pre-40S ribosomal subunit to the cytoplasm, facilitating the final stages of ribosome assembly.
• The protein localizes primarily to the nucleolus, consistent with its role in early rRNA processing events.
• UniProtKB keywords highlight NOP14 as a phosphoprotein, suggesting potential regulation by phosphorylation during ribosome biogenesis.
• Alternative splicing of the NOP14 gene generates multiple transcript variants, which may contribute to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1–240) of human NOP14, making this antibody suitable for detecting N-terminal epitopes.
• For Western blotting, a band migrating at approximately 98 kDa is expected in human cell or tissue lysates; consider using appropriate loading controls and validating in your specific sample system.
• In ELISA applications, optimization of coating antigen and antibody dilution is recommended to achieve optimal signal-to-noise ratios.
• Due to the polyclonal nature, this antibody may detect multiple isoforms or degradation products; if needed, confirm specificity by siRNA knockdown or overexpression in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, research labs, and clinical institutions globally. This anti-NOP14 polyclonal antibody supports investigations into ribosome biogenesis and nucleolar function. Beyond this product, CamelBio offers comprehensive supply solutions—including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets—streamlining your development workflow from concept to clinic.