Anti-NOP10 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9NPE3

Core Product Specifications and Parameters

Biological Background: NOP10 Function and Localization

• NOP10 (Nucleolar protein 10), also known as NOLA3 and DKCB1, is a core subunit of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex.
• This complex is essential for ribosome biogenesis and telomere maintenance, catalyzing the pseudouridylation of rRNA—a modification where uridine is isomerized to pseudouridine (PubMed:32554502). Related references: PMID:32554502
• NOP10 localizes predominantly to the nucleolus and Cajal bodies within the nucleus, aligning with its role in RNA modification and processing.
• It is implicated in the processing and intranuclear trafficking of TERC, the RNA component of telomerase, thereby contributing to telomere homeostasis.
• Mutations in the NOP10 gene are linked to dyskeratosis congenita, a multisystem disorder that can include cataracts, deafness, and other clinical features, highlighting its importance in human disease.
• Additional keywords from UniProt include: 3D-structure, disease variant, reference proteome, ribonucleoprotein, ribosome biogenesis, rRNA processing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-64 of human NOP10, a region that shares high sequence homology with mouse and rat, supporting the observed cross-reactivity.
• For western blot, the predicted molecular weight of NOP10 is approximately 8 kDa. Consider using a 15% SDS-PAGE gel or a gradient gel and a PVDF membrane with 0.2 µm pore size to ensure efficient transfer of this small protein.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval with citrate buffer, pH 6.0) and primary antibody dilution for each tissue and fixation protocol.
• For immunoprecipitation (IP), confirm compatibility with your chosen lysis buffer and antibody concentration; pre-clearing may reduce non-specific binding.
• In ELISA, perform checkerboard titrations to determine optimal coating antigen and antibody concentrations; the peptide immunogen suggests potential use in direct or sandwich ELISA formats.
• Since NOP10 functions in the nucleus/nucleolus, subcellular fractionation may enhance detection in western blot or IP assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined, one-stop channel for IVD raw materials. For ribosome biogenesis and telomere biology research, we offer validated anti-NOP10 monoclonal antibodies and complementary reagents in analytical to bulk scales. Our portfolio also includes sourcing of rare targets and customized antibody development to accelerate your assay development from concept to clinic.