Anti-NONO/p54nrb Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15233

Core Product Specifications and Parameters

Biological Background: NONO/p54nrb Function and Localization

• NONO/p54nrb is a DNA- and RNA-binding protein that binds double-stranded octamer DNA sequences, as well as single-stranded DNA and RNA, playing a central role in nuclear processes.
  Related references: PMID:11525732, PMID:12403470, PMID:26571461
• It functions as a heterodimer with SFPQ to participate in pre-mRNA splicing and interacts with U5 snRNA via a purine-rich sequence.
  Related references: PMID:12403470
• Along with PSPC1, NONO is essential for the formation of nuclear paraspeckles, subnuclear bodies involved in RNA retention and editing.
  Related references: PMID:22416126
• The SFPQ-NONO heteromer plays a role in DNA double-strand break repair by non-homologous end joining (NHEJ), stabilizing paired DNA ends and cooperating with the Ku70/Ku80 dimer.
  Related references: PMID:15590677
• NONO contributes to transcriptional regulation; for example, it forms a complex with SFPQ and NR5A1 to control CYP17 promoter activity in response to cAMP.
  Related references: PMID:11897684
• It represses the CLOCK-BMAL1 heterodimer to regulate circadian rhythms, with important roles in synaptic RNA regulation and GABAergic synapse organization.
• NONO participates in the innate immune response to DNA viruses by assembling into the HDP-RNP complex, which facilitates IRF3 phosphorylation and activates the cGAS-STING pathway, thereby modulating antiviral immunity.
  Related references: PMID:28712728, PMID:30270045
• Subcellular localization is predominantly nuclear, including nucleolus, nuclear speckles, and chromosomes; it is broadly expressed across tissues, with presence in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, and breast tumor cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 372-471) of human NONO/p54nrb, offering target specificity; validate for expected 54 kDa band in WB using lysates from human, mouse, or rat cells.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization to reveal nuclear speckles and paraspeckles; consider co-staining with SFPQ antibody to visualize heterodimer localization.
• In IHC-P applications, confirm staining patterns with appropriate tissue controls and antibody titration, focusing on nuclear expression in known positive tissues like brain or liver.
• Utilize ELISA for quantitative protein detection or screening; note that reactivity has been demonstrated for human, mouse, and rat species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your NONO/p54nrb research and assay development needs, we offer validated antibodies (monoclonal and polyclonal), bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to streamline your workflow and accelerate translation from bench to diagnostic application.