Anti-NONO/p54nrb Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15233

Core Product Specifications and Parameters

Biological Background: NONO/p54nrb Function and Localization

• NONO/p54nrb, also known as p54nrb, NMT55, NRB54, or PPP1R114, is a 54 kDa nuclear RNA- and DNA-binding protein.
• It binds double-stranded DNA at octamer sequences and also interacts with single-stranded DNA and RNA via distinct binding sites. Related references: PMID:11525732, PMID:12403470, PMID:26571461
• As a heterodimer with SFPQ, it participates in pre-mRNA splicing and associates with U5 snRNA. Related references: PMID:12403470
• Together with PSPC1, NONO is essential for nuclear paraspeckle formation, which sequesters defective RNAs. Related references: PMID:22416126
• The SFPQ-NONO complex facilitates non-homologous end joining (NHEJ) DNA double-strand break repair and stabilizes DNA ends. Related references: PMID:15590677
• NONO regulates transcription, including repressing the circadian CLOCK-BMAL1 heterodimer (by similarity) and activating intracisternal A particle promoters.
• In innate immunity, NONO assembles into the HDP-RNP complex to promote IRF3 phosphorylation and cGAS-STING pathway activation, with a specific role in detecting HIV-2 infection. Related references: PMID:28712728, PMID:30270045
• It is widely expressed in tissues and localizes to the nucleus, nucleolus, nuclear speckles, and chromosomes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 300 amino acids of human NONO/p54nrb. This region may contain common isoforms; consider verifying specificity in your target tissue or cell line by knockdown or knockout controls.
• For Western blotting, the predicted molecular weight is ~54 kDa. Use appropriate gel percentage and transfer conditions. Blocking and antibody dilution should be optimized for the sample type.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization methods may influence detection; test citrate or EDTA-based retrieval and Triton X-100 permeabilization.
• For ELISA applications, coating antigen and detection conditions should be empirically determined.
• Cross-reactivity is reported with human, mouse, and rat; however, sequence homology across other species should be checked for your model.
• As a polyclonal antibody, batch-to-batch consistency may vary; consistent results can be achieved by using the same lot for comparative experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to high-quality IVD raw materials, including validated polyclonal and monoclonal antibodies tailored for biomarker development and research assays. Our NONO/p54nrb antibody supports investigations into RNA splicing, DNA repair, paraspeckle biology, and innate immune signaling—key areas in oncology, neurology, and infectious disease research. Beyond antibodies, we offer a comprehensive portfolio of ancillary reagents and technical services to accelerate your project from early-stage discovery to clinical application.