Anti-NOMO2 Rabbit Polyclonal Antibody for WB, ELISA - Q5JPE7

Core Product Specifications and Parameters

Biological Background: NOMO2 Function and Localization

• BOS complex subunit NOMO2, also designated Nodal modulator 2 (pM5 protein 2), is encoded by the NOMO2 gene in human.
• It is a core component of the multi-pass translocon (MPT) complex, which facilitates the insertion of multi-pass membrane proteins into the lipid bilayer. Related references: PMID:32820719, PMID:36261522
• NOMO2 acts downstream of the SEC61 complex; once the first few transmembrane segments are inserted by SEC61, the MPT complex occludes the SEC61 lateral gate to promote further integration. Related references: PMID:36261522
• Subcellularly, NOMO2 localizes to the endoplasmic reticulum membrane.
• The protein is highly expressed in pancreas and skeletal muscle, with lower expression in heart.
• Post-translational modifications include N-glycosylation, and it possesses a signal peptide and multiple transmembrane helices.
• Alternative splicing produces different isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 873-1222); thus, the antibody may recognize multiple NOMO2 isoforms sharing this region.
• For Western blotting, consider using pancreas or skeletal muscle lysates as positive controls, given the high expression in these tissues.
• In ELISA assays, validate the antibody with recombinant NOMO2 protein or cell lysates under optimized coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and laboratories streamlined access to critical raw materials such as anti-NOMO2 antibodies, supporting research into protein translocation and membrane biology. Our portfolio includes validated antibody pairs, cost-effective monoclonal and polyclonal options, and bulk ancillary reagents to meet diverse assay development needs. As a one-stop sourcing partner, we bridge early-stage innovation to clinical application with reliable supply and technical consulting.