Anti-NOMO1 Polyclonal Antibody for WB, IHC-P, ELISA - Q15155

Core Product Specifications and Parameters

Biological Background: NOMO1 Function and Localization

• NOMO1 is the BOS complex subunit Nodal modulator 1, also known as pM5 protein, with a molecular weight of approximately 134 kDa.
• It is a component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. Related references: PMID:32820719, PMID:36261522
• The MPT complex takes over after the SEC61 complex, occluding the lateral gate of SEC61 to promote insertion of subsequent transmembrane regions. Related references: PMID:36261522
• NOMO1 localizes to the endoplasmic reticulum membrane.
• It is expressed in colon tumor tissue and in adjacent normal colonic mucosa.
• The protein is a glycoprotein and phosphoprotein, containing a signal peptide and multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 700–1000) of human NOMO1. This region may be less conserved across species; verify specificity in your experimental system.
• For Western blot (WB) applications, consider optimizing dilution based on preliminary experiments; typical starting dilutions for rabbit polyclonal antibodies range from 1:500 to 1:2000.
• For immunohistochemistry (IHC-P), ensure proper antigen retrieval and include appropriate positive and negative controls.
• Due to reported cross-reactivity with mouse and rat, this antibody can be used in rodent tissue or cell line studies, but validate expression levels in your models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing diagnostic developers with reliable antibodies, protein reagents, and technical support from concept to clinic. This NOMO1 antibody supports research into endoplasmic reticulum biology and membrane protein insertion pathways. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.